Site-specific Incorporation of Phosphoserine
PROMEDIGEN’s core technology enables production of disease-specific PPI models by selective phosphorylation and custom-made PTMs.
① Evolved SepRS produces phosphoseryl tRNA
② Engineered EF-Sep delivers phosphoseryl tRNA to the ribosome
③ Phosphoserine is inserted into a selected position of the protein during translation
④ Now, this phospho-protein is ready to interact with a specific binding partner, generating a custom-made PPI, which can be isolated and purified to enable high-throughput screening.